Signature Benefits

• Mass range up to m/z 3000
• Scan speeds: up to 5000 u/s
• Over 300 SRM transitions per second
• Highest selectivity with enhanced resolution (H-SRM)
• Simultaneous quantitation and confirmation with QED-MS/MS
• PPINICI™-pulsed positive ion negative ion chemical ionization for sample screening

With the TSQ Quantum GC Thermo Fisher Scientific continues the successful and well known TSQ™ triple quadrupole mass spectrometer series. The TSQ Quantum GC incorporates the field-proven sensitive and rugged DuraBright™ source, which, in conjunction with our precision hyperbolic quadrupoles that provide the highest ion transmission and peak shape, and detection technology, results in the most sensitive triple quadrupole instrument available today.

State-of-the-art electronics and comprehensive diagnostics are hallmarks of our instruments. You have total instrument control at your fingertips through the easyto- use Xcalibur™ software. With these industry-leading features, the TSQ Quantum GC, with the TRACE GC Ultra™ and the TriPlus™ Autosampler, defines the new standard of excellence in GC-MS/MS analysis.

DuraBright Source

• DuraBright source allows detection of low-level analytes in challenging matrices and provides unparalleled high uptime along with increased sensitivity
• No tools required for source maintenance
• Durable materials guarantee long life and simple maintenance
• Exchangeable ion volumes
• Standard EI and CI or EI/CI ion volumes, maximizing performance in EI, positive ion CI, and negative ion CI
• Computer-controlled CI reagent gas flow control for high quantitative accuracy and day-to-day calibration stability
• Unique electron lens isolates filament from contaminants of the source, increases lifetime, and improves ionization efficiency
• Electron beam collimating magnets further increase ionization efficiency
• Electron energy adjustable between 0 and 140 eV
• Emission current up to 1000 μA
• Independently controlled heating from 125-300°C for stable operation and superior chromatographic integrity
• GC interface temperature up to 350°C

Triple Quadrupole Mass Analyzer

• Mass range of m/z 10-3000
• Patented HyperQuad™ mass analyzers provide superior and unique combination of resolution and sensitivity
• H-SRM (Q1, Q3) at 0.4 FWHM
• 90° high-efficiency square quadrupole
• Collision cell with noise-reducing geometry
• CID gas pressure programmable through the software
• Variable peak width selection in all scan modes
• Scan rate of 5,000 u per second

Vacuum System

• Unique close-coupled triple inlet turbo molecular pumping 270 L/s
• Two stages of pumping provide optimal vacuum throughout GC/MS/MS analyzer
• Single mechanical pump 30 L/min, floorstanding

Detection System

• Patented detection system uses a fast switching (<95 ms) post-acceleration conversion dynode with ±15 kV applied voltage
• Off-axis continuous dynode electron multiplier with increased dynamic range
• System integrated electron multiplier eliminates field emission and microphonic noise
• Centroid or profile data acquisition modes
• PPINICI option to acquire positive ion CI and negative ion CI spectra in alternating scans

Scan Functions

• Highly sensitive Full-Scan MS in Q1 or Q3
• Selected Ion Monitoring (SIM) in Q1 or Q3
• Selected Reaction Monitoring (SRM) for the most demanding quantitative assays
• Product Ion Scan
• Precursor Ion Scan
• Neutral Loss Scan

Advanced Data-Dependent™ Experiments

• Available from all scan functions
• Dynamic Exclusion™ allows acquisition of MSn spectra from lower intensity ion species
• Polarity switching capabilities
• AutoSIM
• Quantitation-enhanced Data-dependent MS/MS (QED-MS/MS) for simultaneous compound confirmation and quantitation
• Reverse Energy Ramp MS/MS spectra (RER) gives information rich MS/MS spectra for solid compound identification

LC-MS Options

• Ion Max™ API source
• Enhanced sensitivity and ruggedness
• Sweep gas reduces chemical noise
• Optimal 60-degree spray angle for best sensitivity and ruggedness
• Interchangeable ESI and APCI ionization probes
• APPI/APCI combination probe
• Removable ion transfer tube provides vent-free maintenance
• High temperature, self-cleaning APCI heater employing state-of-the-art ceramic heater technology
• X, Y, and Z probe positioning adjustments for all ionization probes
• Automatic source recognition for ease of use and simplified data logging
• Automated valve for making manual loop injections or diverting LC flow stream to waste
• Automated infusion with syringe pump
• Automated loop injection from syringe pump for analyte optimization

Data Acquisition

• Real-time, high-speed, digital signal processing with dedicated AD SHARC DSP
• Digital sampling rate up to 195,000 samples per second
• High-resolution centroiding Instrument Diagnostics
• Graphical diagnostics for all power supplies, electronic circuits and pumping system
• Remote access allows Thermo Fisher Scientific engineers to troubleshoot via modem
• Electronic logbook of diagnostic results

Data System

• Xcalibur processing and instrument control software
• Autotune
• Autocalibration
• Data system control of all instrument parameters
• Superior comprehensive instrument diagnostics
• Automated optimization of all instrument parameters including gas pressures and collision energy within an experiment

Application specific Software

• LCQUAN™ quantitation software supports 21 CFR Part 11 compliance
• NetWorks™-automated metabolite identification using spectral trees
• Mass Frontier™-spectral interpretation and classification software to identify unknowns
• NIST library, now including MS/MS spectra
• Wiley library
• Pfleger-Maurer-Weber library
• Pesticide library

GC/MS Specifications

All evaluations performed using an Rtx®- 5MS, 15 m× 0.25 mm ID (0.25 micron film thickness) fused silica capillary column.

• Electron Ionization Full Scan: Hot splitless injection of 1 μL of a 1 pg/μL (3.67 fmol/μL) standard of octafluoronaphthalene (OFN) in iso-octane will produce a minimum signal-to-noise of 100:1 for m/z 272 when scanning from 200-300 Da at five scans per second.
• Electron Ionization SIM: Hot splitless injection of 1 μL of a 50 fg/μL (183.8 amol/μL) standard of octafluoronaphthalene (OFN) in iso-octane will produce a minimum signal-to-noise of 50:1 for m/z 272 when operated in selected ion monitoring mode (SIM) at five scans per second. Q1 or Q3 resolution set to 0.7 Da FWHM. If required this specification can also be performed with Q1 or Q3 resolution set to 0.4 FWHM.
• Positive Chemical Ionization Full Scan Hot splitless injection of 1 μL of a 10 pg/μL (54.9 fmol/μL) standard of benzophenone in n-heptane will produce a minimum signal-to-noise of 10:1 for the protonated molecular ion at m/z 183 when scanning from 80-230 Da at 2 scans per second with methane as the reagent gas.
• Positive Chemical Ionization SRM Hot splitless injection of 1 μL of a 2 pg/μL (11.0 fmol/μL) standard of benzophenone in n-heptane will produce a minimum signal-to-noise ratio of 200:1 for the transition of the protonated molecular ion at m/z 183 to the fragment ion at m/z 105 when operated in selected reaction monitoring mode (SRM) at five scans per second with Q1 and Q3 resolution set to 0.7 Da FWHM with methane as the reagent gas. If required this specification can also be performed with Q1 resolution set to 0.4 FWHM.
• Negative Chemical Ionization Full Scan Hot splitless injection of 1 μL of a 1 pg/μL (3.67 fmol/μL) standard of OFN in isooctane will produce a minimum signal-to noise of 1000:1 for m/z 272 when scanning from 200-300 Da at five scans per second with methane as the reagent gas.
• Negative Chemical Ionization SIM Hot splitless injection of 1 μL of a 10 fg/μL (36.7 amol/μL) standard of OFN in iso-octane will produce a minimum signal-to-noise of 50:1 for m/z 272 when operated in selected ion monitoring mode (SIM) at five scans per second. Q1 or Q3 resolution set to 0.7 Da FWHM with methane as the reagent gas. If required this specification can also be performed with Q1 resolution set to 0.4 FWHM

Optional LC/MS Specifications

• Electrospray (ESI) at Unit Resolution and H-SRM (0.4 FWHM) A 5 μL loop injection of a 2 pg/μL (3.250 fmol/μL) reserpine solution at a flow rate of 400 μL/min 50/50 IPA/water will produce a minimum signal-to-noise ratio of 100:1 for the transition of the protonated molecular ion at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 Da FWHM. If required this specification can also be performed with Q1 resolution set to 0.4 FWHM.
• Atmospheric Pressure Chemical Ionization (APCI) and Atmospheric Pressure Photoionization (APPI) at Unit Resolution and H-SRM (0.4 FWHM) A 5 μL loop injection of a 2 pg/μL (3.250 fmol/μL) reserpine solution at a flow rate of 1 mL/min 50/50 IPA/water will produce a minimum signal-to-noise ratio of 100:1 for the transition of the protonated molecular ion at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 Da FWHM. If required this specification can also be performed with Q1 resolution set to 0.4 FWHM.