-HCD (Higher Energy Collisional Dissociation) Powered by patented Thermo Scientific

Orbitrap™ technology, the Exactive™ provides fast, sensitive, reliable, and confident detection and identification of compounds in complex mixtures while maintaining full compatibility with U-HPLC and fast chromatography.

The Exactive supports screening applications, from routine compound identification to the most demanding analysis of trace levels components in complex mixtures. In addition, the Exactive allows an alternate positive-negative scan mode of operation.

The optional HCD collision cell adds more functionality to the bench-top system by providing ‘All Ion Fragmentation’ while maintaining high resolution, accurate mass and high sensitivity.

Hardware Specifications

Exactive LC-MS Instrument Ion Max™ API Source

• Enhanced sensitivity and ruggedness
• Sweep Gas™ reduces chemical noise
• 60° interchangeable ion probe orientation
• Removable metal ion capillary tube provides vent-free maintenance

Transfer Ion Optics

• Advanced ion guides
• High stability and ion transmission efficiency

Vacuum System

• Differentially pumped vacuum system with:

- Rotary vane fore vacuum pump and two aircooled 300 L turbomolecular pumps

- Final vacuum under operating conditions < 1 x 10-9 Torr

- Vacuum control by active Pirani gauge and two cold ion gauges

Orbitrap Mass Analyzer

• Gas (nitrogen) filled C-Trap
• Highly efficient ion transfer optics to

Orbitrap analyzer

• Straight multipole collision cell for HCD (optional)
• Orbital trap with 5 kV central electrode voltage
• Low-noise image current pre-amplifier
• 16-bit signal digitization
• Ultra fast real-time data acquisition and instrument control system
• Fully automated calibration via instrument control software

Performance Characteristics

• Mass Range m/z 50-4000
• Resolution 100,000 at m/z 200 at a scan rate of 1 Hz, minimum resolution 10,000 at m/z 200 at a scan rate of 10 Hz
• Mass Accuracy < 5 ppm with external calibration, < 2 ppm using internal calibration
• Polarity Switching One full cycle in < 1 sec (one full scan positive mode and one full scan negative mode at resolution setting of 10,000)
• Sensitivity A flow injection of 500 fg of Buspirone giving a S/N of better than 10:1 for the selected mass chromatogram of the [M+H]+ ion (m/z = 386.2551)
• Dynamic Range > 4,000 within a single scan with guaranteed specified mass accuracy
• MS Scan Power Full scan and ‘All Ion Fragmentation’ by HCD
• Scan Rate 1 - 10 Hz
• Analog Inputs One (1) analog input (0-1 V), One (1) analog (0-10 V)

Options

• ESI probe compatible with liquid flow rates of < 1 μL/min to 1 mL/min without splitting (required)
• Nanospray source supports static packed tip and dynamic nanospray experiments, compatible with liquid flow rates of 50 nL/min to 2 μL/min*
• APCI source compatible with liquid flow rates of 50 μL/min to 2 mL/min without splitting
• APCI/APPI source compatible with liquid flow rates of 50 μL/min to 2 mL/min without splitting
• H-ESI probe with Dual Desolvation Zone technology
• Metal needle option for high- and lowflow analyses

Software Features

Data System

• High performance PC with Intel® Pentium® microprocessor
• High resolution LCD color monitor
• Microsoft Windows® XP operating system
• Microsoft Office XP software package
• Thermo Scientific Xcalibur™ processing and instrument control software
• Thermo Scientific Pathfinder − software to enable expert and walk-up instrument use for compound screening and identification

Operation Modes

• High resolution accurate mass scans at high scan rates
• All Ion Fragmentation’ in the HCD collision cell with high resolution accurate mass detection (optional)
• Source fragmentation of all ions in the skimmer region of the ion source
• Alternating positive/negative ion scanning while maintaining high resolution accurate mass data acquisition

Optional Application-Specific

Software

• Thermo Scientific ToxID™ is an easy-to use software that substantially simplifies LC-MS/MS toxicology screening workflows for clinical and forensic laboratories
• Thermo Scientific MetWorks™ − automated metabolite identification using spectral trees and accurate mass data
• Mass Frontier™ − predictive fragmentation software for spectral interpretation and classification software to identify unknowns

Exclusive Technologies

• Unique, patented Automatic Gain Control (AGC™) ensures that the Orbitrap is always filled with the optimum number of ions for all scan speeds
• Variable collision energy for HCD fragmentation technique allows userdefined variation of the collision energies in MS/MS experiments
• Collision energy profiling using different collision energies for HCD fragmentation technique in one instrument method