Hardware Features

Ion Max API Source

• Enhanced precision and ruggedness
• Sweep gas reduces chemical noise
• Optimal 60-degree spray angle
• Interchangeable HESI-II and APCI ionization probes
• Removable ion transfer tube provides vent-free maintenance
• High temperature, self-cleaning APCI heater employing state-of-the-art ceramic heater technology
• Dual Desolvation Zone Technology with the HESI-II
• G2 ion optics for improved ion transmission
• X, Y, and Z probe positioning adjustments for all ionization probes
• Automatic source recognition for ease of use and simplified data logging
• Square profile quadrupole ion guide for the highest ion transmission

Triple Stage Quadrupole

• Dual HyperQuad™ precision hyperbolic quadrupole mass analyzers for ultra performance
• Large 6 mm field radius provides high transmission and superior peak shape
• 90-degree square profile quadrupole rod assembly ion guide with noise-reducing geometry
• Software control and automated optimization of collision energy and gas pressure
• Fully automated system calibration, tuning, and compound optimization

Vacuum System

• Four-stage differentially pumped vacuum manifold
• Advanced triple inlet turbomolecular pump integrated with vacuum manifold
• Dual rotary vacuum pump configuration

Detection System

• Off-axis continuous dynode electron multiplier with extended dynamic range
• ±15 kV post-acceleration conversion dynode
• Digital electronic noise reduction
• System integrated electron multiplier eliminates field emission and microphonic noise

Integrated Divert Valve

• Fully automated data system control enables switching the solvent front, gradient end point, or any portion of the HPLC run to waste
• Capable of automatic system optimization by loop injections
• User-definable default state of the valve,either “to waste-load”or “to source-inject”

Integrated Syringe Pump

• Automated infusion and loop injections under full data system control

Source Options

• HESI-II source compatible with liquid flow rates of 1 μL/min to 2 mL/min, without splitting
• APCI/APPI source compatible with liquid flow rates of 50 μL/min to 2 mL/min, without splitting
• Metal needle configurations for low-flowor high-flow analysis
• NanoSpray source supports both static and dynamic nanospray experiments, compatible with liquid flow rates of 50 nL/min* to 50 μL/min
• FAIMS reduces noise and matrix interferences, resulting in improved assay robustness and increased assay sensitivity

Scan Functions

• Highly sensitive full-scan MS in Q1 or Q3
• Selected Ion Monitoring (SIM) in Q1 or Q3
• Timed Selected Reaction Monitoring (SRM) for the most demanding bioanalytical and proteomics assays. Up to 3000 SRMs definable.
• Product Ion Scanning
• Precursor Ion Scanning
• Neutral Loss Scanning
• H-SRM
• Reverse Energy Ramp MS/MS spectra (RER) gives information rich MS/MS spectra for solid compound identification Advanced Data Dependent™

Experiments

• Available from all scan types
• Dynamic Exclusion™ allows acquisition of MSn spectra from lower intensity ion species
• Polarity switching capabilities
• AutoSIM
• AutoLock feature for accurate mass systems
• Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS) for simultaneous compound confirmation and quantitation

System Control

• Powerful embedded computer system with Motorola PowerPC® processor
• Integrated Serial Peripheral Interconnect (SPI) bus for reliable electronic communications between system modules
• Dedicated SHARC® digital signal processor (DSP) for dedicated instrument control
• Fast 100BASE-T Ethernet port for PC-toinstrument communications

Data Acquisition

• Real-time, high-speed, digital signal processing with dedicated SHARC digital signal processor (DSP)
• High-speed analog-to-digital converter (ADC) with 195 kHz sampling rate
• Adjustable scan speeds to 5000 u/s
• High-resolution centroiding

Data System

Instrument Control-Thermo Scientific Xcalibur™

• Xcalibur processing and instrument control software
• Data system control of all instrument parameters
• Superior comprehensive instrument diagnostics
• Automated optimization of all instrument parameters including gas pressures and collision energy within an experiment
• Direct control of multiple vendor LC systems and autosampler configurations through Xcalibur data system software
• High performance PC with Microsoft® Windows® operating system
• 19-inch viewable ultra sharp flat screen display monitor

Optional Application-Specific Software

• LCQUAN™ quantitation software supports 21 CFR Part 11 compliance
• Watson LIMS™-highly specialized protocol-driven Laboratory Information Management System designed to support DMPK/Bioanalytical studies in drug development
• Galileo LIMS™-fully integrated system for in vitro ADME experiments in a single client-server application
• QuickQuan™-high-throughput, automated quantitation software for early drug discovery
• QuickCalc™ software powered by Gubbs Inc. GMSU-quantitation and reporting solution for high-throughput ADME laboratories
• MetWorks™-automated metabolite identification, component detection and predicted fragmentation in one intuitive workplace
• Mass Frontier™-spectral interpretation and classification software for the identification of unknowns

System Specifications

Sensitivity

• Electrospray (HESI) at Unit Resolution A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/ Formic acid will produce a minimum signalto- noise ratio of 500:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 daltons (Da) FWHM.
• Electrospray (HESI) at High Resolution A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signalto- noise ratio of 500:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in Highly Selective Reaction Monitoring mode (H-SRM) with Q1 resolution set to 0.2 Da FWHM and Q3 resolution set to 0.7 Da FWHM.
• Atmospheric Pressure ChemicalIonization (APCI) at Unit Resolution A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/ Formic acid will produce a minimum signalto- noise ratio of 500:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 Da FWHM.
• Atmospheric Pressure Chemical Ionization (APCI) at High Resolution A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signalto- noise ratio of 500:1 for the transition of the protonated molecule at m/z 609.3 to the fragment ion at m/z 195.1 when operated in Highly Selective Reaction Monitoring mode (H-SRM) with Q1 resolution set to 0.2 Da FWHM and Q3 resolution set to 0.7 Da FWHM.

Performance Specifications

Mass Range

• m/z 10-1500 daltons (Da) (TSQ Vantage and TSQ Vantage AM)
• m/z 10-3000 Da (TSQ Vantage EMR)

Resolution

• 7500 (FWHM) at m/z 508 of polytyrosine
• Resolution is continuously adjustable to better than 0.1 Da peak width (FWHM) across the entire mass range

Mass Stability

TSQ Vantage/TSQ Vantage EMR

• Mass assignment will be within ±0.050 Da over a 24-hour period. The laboratory room temperature must be maintained between 15-27°C (59-81°F). The optimum temperature of operation is between 18-21°C (65-70 °F). The room temperature may not change by more than 5°C (9 °F) during this period.

TSQ Vantage AM

• Mass assignment will be within ±0.025 Da over a 24-hour period. The laboratory room temperature must be maintained between 15-27°C (59-81°F). The optimum temperature of operation is between 18-21°C (65-70 °F). The rate of change in temperature may not exceed 2°C per hour (3.6 °F/hr) and not by more than 5°C (9 °F) during this period.

Mass Accuracy-TSQ Vantage AM

• Infusion of a mixture of polyethylene glycols (PEGs) of average molecular weights 200, 400, 600, and 1000 at 50 pmol/μL produces 27 ammoniated PEG ions from 124 to 1268 Da. Accurate mass data is generated on each of the 25 ions from 168 to 1224 Da using the neighboring peaks as internal lock masses. The mass of each ion is determined from the average of up to 100 scans and the error between the expected mass and the measured mass is expressed in mmu and ppm. The root mean square (RMS) average is computed from the errors of the 25 individual ions. The RMS error will be less than or equal to 5 ppm.

Installation Requirements

Power

• One 230 Vac ±10%, 50/60 Hz at 16 A minimum
• Four 120 Vac + 6-10%, 50/60 Hz at 20 A or four 230 Vac ±10%, 50/60 Hz at 13 A
• Earth ground hardwired to main panel
• Free from voltage variations above or below the recommended operating range

Gas

• Collision gas: 99.995% pure Argon
• Collision gas supply pressure: 135 ±70 kPa (20 ±10 psig)
• Sheath/aux/sweep gas: 99% pure Nitrogen
• Sheath/aux/sweep gas supply pressure: 690 ±140 kPa (100 ± 20 psig)
• Maximum sheath gas consumption: ~ 20 L/min

Environment

• Functional temperature range: 15°C to 27°C (59°F to 81°F)
• Optimal temperature range: 18°C to 21°C (65°F to 70°F)
• TSQ Vantage heat output: 2,300 W (8,000 Btu/h)
• Total system heat output: 4,420 W (15,380 Btu/h)
• Particulate matter: < 3,500,000 particles per cubic meter of air (< 100,000 particles of > 5 μm diameter per cubic foot of air)
• Relative humidity: 20% to 80%, without condensation
• Floors must be free of vibration